NEBNext ARTIC products for Illumina® and Oxford Nanopore Technologies® sequencing
NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA
In response to the Corona pandemics and related critical need for reliable and accurate methods for SARS-CoV-2 sequencing, New England Biolabs (NEB) has developed NEBNext ARTIC kit options for short and long read sequencing on the Illumina and Oxford Nanophore Technologies, respectively.
The development of these kits has been based on the original work of the ARTIC Network. The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach.
NEBNext® ARTIC SARS-CoV-2 Companion Kit (#E7660)
This kit is compatible with sequencing on the Oxford Nanopore Technologies (ONT) platform and is used alongside kits from ONT to generate libraries, that can be sequenced on any ONT instrument in 2-24 hour run times.
- Library inserts in the 400 bp range
- Effective with a wide range of viral genome inputs (10-10,000 copies)
- Express protocol option available, with reduced cleanup steps recommended for inputs of ≥ 100 copies of the (SARS-CoV-2) viral genome.
NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®) (#E7650)
This kit produces Illumina-compatible libraries with library inserts, compatible with 2 x 250 sequencing on Illumina instruments.
- Library inserts in the 400 bp range
- Effective with a wide range of viral genome inputs (10-10,000 copies)
- No requirement for amplicon normalization prior to library preparation
- Includes NEBNext Sample Purification Beads (SPRIselect®)
- Express protocol option available with reduced cleanup steps recommended for inputs of ≥ 100 copies of the (SARS-CoV-2) viral genome.
NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) (#E7658)
This kit incorporates enzymatic cDNA fragmentation and produces Illumina-compatible libraries with library inserts, compatible with 2 x 75 sequencing on Illumina instruments.
- Library inserts in the 150 bp range bp range.
- Effective with a wide range of viral genome inputs (10-10,000 copies).
- No requirement for amplicon normalization prior to library preparation.
- Optional use control human primers provided.
- Includes NEBNext Sample Purification Beads (SPRIselect®).
- Express protocol option available, with reduced cleanup steps recommended for inputs of ≥ 100 copies of the (SARS-CoV-2) viral genome.
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