Introducing NEBNext UltraExpress™ Library Prep Kits for DNA & RNA 

The NEBNext UltraExpress Library Prep Kit is the latest generation of NEBNext DNA and RNA library prep, with a fast, streamlined workflow that enables the creation of high-yield, high-quality libraries. 

How fast? 

• DNA: < 2 hours
• RNA: 3 hours

NEBNext UltraExpress™ DNA Library Prep Kit / NEBNext UltraExpress™ FS DNA Library Prep Kit
#E3325S/L / #E3340S/L  

NEBNext UltraExpress is the next generation of NGS library prep, and the NEBNext UltraExpress DNA Library Prep Kit offers a one-size-fits-all protocol (within the stated input range) for simplified and streamlined library prep, from sample to high-quality library in under two hours! The NEBNext UltraExpress Library Prep Kits for DNA is available with (NEB-E3340) and without (NEB-E3325) enzymatic fragmentation. 

• Fast workflow (< 2 hours)  
• Fewer steps and consumables  
• Fewer cleanups  
• High-quality libraries from a wide input range (10 - 200 ng pre-sheared DNA)  
• Single protocol for all inputs 
• Automation friendly: protocol designed by NEB is available on Beckman i7

View or download extensive performance data in the ata Supplement for NEB-E3325 and NEB-3340. 

NEBNext UltraExpress™ RNA Library Prep Kit
#E3330S/L 

The NEBNext UltraExpress RNA Library Prep Kit is the latest generation of NEBNext RNA library prep, and it has a fast, streamlined workflow. With a 3-hour library prep protocol, the kit enables creation of high-quality RNA libraries in a single day, in conjunction with mRNA enrichment or rRNA depletion kits.  

• Fast workflow (3 hours) 
• Fewer steps and consumables  
• Fewer cleanups  
• Single protocol for all input amounts 
• High-quality libraries from a broad input range: 25 - 250 ng total RNA 
• Automation friendly: protocol designed by NEB is available on Beckman i7

View or download extensive performance data in the  Data Supplement.