In vivo Functional Grade Antibodies
by Tram Nguyen
Preclinical Functional Grade
The Assay Genie in vivo functional-grade antibodies are high purity, low endotoxin monoclonal antibodies that can be used in translational & preclinical animal studies as well as in vitro functional assays. These antibodies have the highest quality standards in the industry with no detectable leachable protein A or aggregates, extremely low levels of endotoxin and up to ≥ 98% purity.
The Assay Genie in vivo antibodies are produced in a cGMP and ISO 9001:2015 / ISO 13485:2015 certified facility & can be used for injection into animals on account of being pathogen-free (IDEXX Impact I PCR mouse pathogen profile).
This range of low- and ultra-low endotoxin antibodies have been tested for use in functional studies such as co-stimulation, depletion, blocking & neutralization experiments as well as applications such as ELISA, FC, ICC, IHC-PE, IHC-FF, IP, WB, ELISpot, CODEX® & CyTOF®.
The antibodies come in flexible pack sizes including 1mg, 5mg, 25mg, 50mg & 100mg pack sizes with custom packaging available on request too.
The catalog includes top targets such as immune checkpoint antibodies like PD-1 [clone RMP1-14], PD-L1 (B7-H1) [clone 10F.9G2], and CTLA-4 [9H10], as well as key targets for immune cell depletion e.g CD4, CD8 and many more.
Specifications | in vivo mAbsLow Endotoxin | in vivo mAbsUltra Low Endotoxin |
| Binding validation determined by WB, FC or ELISA | Yes | Yes |
| Endotoxin level determined by the LAL method | ≤ 1.0 EU/mg | ≤ 0.5 EU/mg |
| Antibody aggregation screening by analytical SEC | ≥ 95% monomer | ≥ 98% monomer |
| Purity by SDS PAGE | ≥ 95% | ≥ 98% |
| Formulation for in vivo use | No Preservatives No stabilizers No carrier protein Sterile PBS pH 7.2 - No K+ or Ca2+ Concentration: > 5mg/mL | No Preservatives No stabilizers No carrier protein Sterile PBS pH 7.2 - No K+ or Ca2+ Concentration: > 5mg/mL |
| Murine pathogen Screening | Not applicable, see Ultra Low Endotoxin functional grade antibodies | Pathogen tested (IMPACT1) |
| Applications | In vivo functional studies and may be used ffor WB, FC, IF or IHC | |
| Product preparation | Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G. to assure extremely low levels of endotoxins, leachable protein A or aggregates. | |
| Storage and handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 1 month, 2 to 8 °C under sterile conditions, as supplied. • 12 months, -70 °C under sterile conditions. | |
Want to know more? Reach out to Tram!
