Shearing technologies
Shearing instruments for successful sample preparation. Diagenode provides several types of shearing devices to precisely meet the requirements of your application.
Bioruptor Pico®
All-in-one shearing system capable of shearing samples from 150 bp to 1 kb.
Bioruptor Plus®
Unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity.
Megaruptor 3® + HT version
The Megaruptor® 3 was designed to provide the best experience with the fragmentation of DNA from 5 kb to 100 kb.
Megaruptor 2®
Fragmentation of DNA from 5 kb - 100 kb, up to 8 samples in parallel.
COMPARISON TABLE:
| Model | Bioruptor Pico® | Bioruptor Plus® | One® | Megaruptor 3® | Megaruptor 2® |
|---|---|---|---|---|---|
| Description | Best suited for ChIP-seq and NGS sample preparation | Best suited for cell and tissue sample preparation | The compact bench top device for optimal sample preparation | Shearing device for long read sequencing applications | Shearing device for long read sequencing applications |
| Key applications |
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| Shearing range | From 5 to 100 kb | From 3 to 75 kb | |||
| DNA input | Up to 75 µg | Up to 20 µg | |||
| Throughput | 16 samples (for 0.2 ml tube holder) 12 samples (for 0.65 ml tube holder) 6 samples (for 1.5 ml tube holder) 6 samples (for 15 ml tube holder) |
12 samples (for 0.5 ml tube holder) 6 samples (for 1.5 ml tube holder) 6 samples (for 15 ml tube holder) 3 samples (for 50 ml tube holder) |
1 sample | 1 – 8 samples in parallel | 1 – 2 samples in series |
| Recommended volumes | 20 - 100 µl (0.2 ml Bioruptor tubes) 100 µl (0.65 ml Bioruptor tubes) 100 - 300 µl (1.5 ml Bioruptor tubes) 300 µl - 2 ml (15 ml Bioruptor tubes) |
100 µl (0.5 ml Bioruptor tubes) 100 - 300 µl (1.5 ml Bioruptor tubes) 300 µl - 2 ml (15 ml Bioruptor tubes) 2 - 20 ml (50 ml tubes) |
20 µl and 50 µl | 65 – 500 µl | 50 – 400 µl |
| Temperature controlled | yes | yes | yes |
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