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Well regarded as top performers for membrane protein structural biology since the late 1980s, Anatrace products are chosen for their uniquely pure molecules – and the exacting chemistry behind them. From compounds designed to disrupt cell membranes to products that stabilize or solubilize unstable macromolecules and proteins, Anatrace is constantly raising the bar. Your structural studies, functional biology work, or chemical synthesis project will yield incredibly reliable results, which is exactly why publications have cited Anatrace an average of over 500 times a year, just in the last 5 years.
Anatrace product range
Anatrace detergents are grouped into seven broad categories:
In addition to offering highly purified maltosides and glucosides, Anatrace also tailors these popular molecules into useful derivatives to meet your specific needs, including Anagrade® and Sol-Grade®, Low or High Alpha anomer, as well as Deuterium, NG Class, Sulfur, and Selenium forms.
Whether you are working with membrane-bound proteins or trying to find ways to stabilize macromolecules, lipids have become valuable tools. While lipids also have the same general structure as detergents— a polar hydrophilic head group and a nonpolar hydrophobic tail—lipids differ from detergents in the shape of the monomers, in the type of aggregates formed in solution, and in the concentration range required for aggregation. Anatrace phospholipid analogs offer lipid alternatives for all of your membrane protein applications.
Anatrace’s products include:
Short Chain Lipids, or PCs, PGs, and Pes
High-purity detergents, lipids, and custom chemistry are the hallmark of Anatrace. Hence, Anatrace also offers a variety of industrial-grade detergents, which are perfect for initial protein extraction and purification of membrane proteins, along with a host of other molecular biology applications. CHAPS, CHAPSO, NDSB-256, and Nonidet P40 Substitute are among the most widely used of Anatrace industrial detergents. Industrial scale manufacturing offers the advantage of lower cost products but at the expense of homogeneity and purity found in other high purity Anatrace products such as Dodecyl Maltoside or Fos-Choline. Industrial detergents are a mixture of different molecular weight components that are primarily the proper derivative. These reliable detergents are commonly found in every lab and are highly suitable for standard applications.
Figure 2. Chemical structure of CHAPSO
From the line of specialty detergents, Amphipol A8-35 is a detergent-free solution for stabilizing membrane proteins in aqueous solutions. The molecule is well characterized and capable of stabilizing membrane proteins in their native structure and preserving functionality. The molecule is quickly becoming a favorite for Single Particle Cryo-EM work as well.
Anatrace also offers deuterated, fluorinated, and selenated detergents. For applications such as NMR, Anatrace used popular detergents and replaced hydrogen atoms with deuterium. Deuterium reduces the signal, making the deuterated-version detergent NMR-silent. Among the detergents growing in popularity are alkyl PEG products and GDN. Anatrace alkyl PEG detergent line is made up of lipophilic chains between 8 and 12 carbons long. In any screening library, alkyl PEG products should be used in conjunction with OG, DM, and DDM. GDN, on the other hand, is quietly becoming a substitute for digitonin. The molecule is non-toxic and more cost-efficient.
Figure 3. Chemical structure of GDN
One of the most challenging aspects of working with membrane proteins is the difficulty of choosing which detergent may be best suited for your application. Membrane proteins evolved in nature into slightly different chemical environments inside their host lipid bilayer. Once extracted, they require a similar environment to remain stable. This makes finding a micelle with similar features challenging.
To select the optimum detergent or combination of detergents and reagents for a particular membrane protein application, often multiple detergents must be tested. Moreover, a detergent that is suitable for extraction may not be useful for storage of the purified protein or functional studies conducted on the purified protein. To aid in the selection process, Anatrace offers a wide variety of solid and solution-based detergent kits to screen for the best fit.
Anatrace kits enable you to screen a large number of detergents quickly and cost-effectively. For example, if you need to find the right glucoside, our Glucopyranoside Detergent Kit (D399-G) contains six different glucosides with tail lengths between 6 (hexyl) and 12 (dodecyl) carbon lengths long. When you’re not quite sure which detergent will work best for your project, Anatrace kits are there to help with multiple 1 gm sample solid kits or ampuled 10% solution kits based around a common theme.
For more info, please contact:
+45 3124 8581