Custom Antibody Conjungation

created on

Custom Antibody Conjungation

Looking for a specific antibody conjugate? CST can conjugate it for you.


Need a conjugated antibody that you don’t see in the Cell Signaling Technology® (CST®) catalog? Save time and avoid pitfalls, like the low yields associated with do-it-yourself (DIY) kits. We can conjugate it for you.


Advantages of CST Custom Conjugation

  • Flexible: Tiered service offerings range from basic antibody conjugation to full validation and stability testing of conjugates.

  • Validated: Conjugates are tested in key applications using biologically relevant cell systems and controls, and verified for physical integrity using size exclusion chromatography.

  • Optimized: Flexible conjugation chemistry, free dye and/or antibody purification, and identification of optimal degree of labeling (DOL) are utilized to ensure the best signal-to-noise ratio.

  • Support: Receive consultation and technical support from the conjugation experts who produce our ready-made conjugates.


Want to see our ready-to-ship antibody conjugates? 

View ready-to-ship in shop 



Conjugation Services available:

Conjugation Services available:

1 Standard validation application method is Flow Cytometry. Alternatives may increase timeline and pricing.
 Standard validation application method is Western Blot. Alternatives may increase timeline and pricing.
 Standard validation application method is IP. Alternatives may increase timeline and pricing.
 Oligonucleotide conjugation offering may be limited for certain IP protected technologies.

Maria Kam Erisken

Reach out to Maria for help with your antibody conjugation request          

+45 2244 8243 

CST Custom Conjugation vs Do-It-Yourself (DIY) Conjugation Kit 

DIY conjugation kits look good on paper, but are you getting your money’s worth? Inefficiencies caused by suboptimal labeling, interference with specificity, and/or destabilization/degradation can negatively affect performance. Another drawback is the loss of antibody during conjugation steps, which can mean finishing with significantly less functional antibody than you started with.

With Custom Conjugation Services from Cell Signaling Technology (CST), you can avoid these time-consuming pitfalls and get the full amount of antibody you pay for.


Comparison of PE-conjugated Antibody in Flow Cytometry

Comparison of PE-conjugated Antibody in Flow Cytometry

Comparison of PE conjugation of Helios (D8W4X) XP® Rabbit mAb using CST custom conjugation method (blue) or conjugated using a competitor’s DIY PE conjugation kit (green). Flow cytometeric analysis of RL-7 (dashed lines), a low-expressing cell line, and Jurkat (solid lines), a high-expressing cell line. The calculated signal-to-noise ratio is shown (above).