CST: News & Products releases

CST is continuously expanding their portfolio to meet your research needs.
Check out some of the latest new products and hot targets, as well as new downloadable resources! 

CAR-T Therapy Research Tools: Detect CAR Surface Expression  

Chimeric antigen receptor (CAR) T therapy is a revolutionary type of adoptive cell immunotherapy, which employs genetic engineering to redirect the specificity of cytotoxic T lymphocytes to a tumor antigen(s) expressed on the surface of tumor cells. 

VHL (E3X9K) Rabbit mAb 
#81292

E3 ligase is important for cancer and hypoxia signaling 

The VHL (Von Hippel-Lindau) protein is a substrate recognition component of an E3 
ubiquitin ligase complex containing Elongin BC (TCEB1 and TCEB2), CUL1, and RBX1.  
Loss of VHL protein function results in a dominantly inherited familial cancer syndrome 
that manifests as angiomas of the retina, hemangioblastomas of the central nervous 
system, renal clear cell carcinomas, and pheochromocytomas. Under normoxic 
conditions, VHL directs the ubiquitylation and subsequent proteasomal degradation 
of the HIF-1α, maintaining very low levels of HIF-1α in the cell. Cellular exposure to 
hypoxic conditions, or loss of VHL protein function, results in increased HIF-1α protein 
levels and increased expression of HIF-induced gene products, many of which are 
angiogenesis factors such as VEGF. Thus, loss of VHL protein function is believed to 
contribute to the formation of highly vascular neoplasias.
 

KEYWORDS: Ubiquitin/Proteasome, Proteolysis, Cancer, Hypoxia signaling 

Validated for: IHC 

TFEB (E4I8R) Mouse mAb
#91767 

Transcription factor important for lysosome and autophagy 
TFEB is a transcription factor of the Myc-related, bHLH leucine-zipper family 
member. TFEB specifically recognizes and binds the CLEAR box (GTCACGTGAC) 
of lysosomal and autophagy genes, resulting in the upregulated expression of 
genes involved in lysosome biogenesis and function, and regulation of autophagy. 
TFEB is activated in response to nutrient deprivation, stimulating translocation to 
the nucleus where it forms homo- or heterooligomers with other members of the 
MiTF subfamily and resulting in upregulation of autophagosomes and lysosomes. 
Recently, it has been shown that TFEB is a component of mTORC1, which 
regulates the phosphorylation and nuclear translocation of TFEB in response 
to cellular starvation and stress. During normal growth conditions, TFEB is 
phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation 
promotes association of TFEB with 14-3-3 family proteins and retention in the 
cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation 
of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it 
increases transcription of CLEAR and autophagy genes. 

KEYWORDS: Lysosome, Autophagy, Nutrient 

Validated for: WB IP IF 

p16 INK4A (BC42) Mouse mAb
#68410 

The VHL (Von Hippel-Lindau) protein is a substrate recognition component of an E3 
ubiquitin ligase complex containing Elongin BC (TCEB1 and TCEB2), CUL1, and RBX1. 
Loss of VHL protein function results in a dominantly inherited familial cancer syndrome 
that manifests as angiomas of the retina, hemangioblastomas of the central nervous 
system, renal clear cell carcinomas, and pheochromocytomas. Under normoxic 
conditions, VHL directs the ubiquitylation and subsequent proteasomal degradation 
of the HIF-1α, maintaining very low levels of HIF-1α in the cell. Cellular exposure to 
hypoxic conditions, or loss of VHL protein function, results in increased HIF-1α protein 
levels and increased expression of HIF-induced gene products, many of which are 
angiogenesis factors such as VEGF. Thus, loss of VHL protein function is believed to 
contribute to the formation of highly vascular neoplasias. 

KEYWORDS: aging, cancer 

Validated for: IHC 

Phospho-HDAC2 (Ser394) (E8O2Z) Rabbit mAb
#69238 

Acetylation of the histone tail causes chromatin to adopt an “open” conformation, 
allowing increased accessibility of transcription factors to DNA. The identification 
of histone acetyltransferases (HATs) and their large multiprotein complexes 
has yielded important insights into how these enzymes regulate transcription. 
HAT complexes interact with sequence-specific activator proteins to target specific 
genes. In contrast, histone deacetylation promotes a “closed” chromatin 
conformation and typically leads to repression of gene activity. Mammalian 
histone deacetylases can be divided into three classes on the basis of their 
similarity to various yeast deacetylases. Inhibitors of HDAC activity are now 
being explored as potential therapeutic cancer agents. 

Picture:  C&R: CUT&RUN was performed with K-562 cells and either Phospho-HDAC2 (Ser394) (E8O2Z) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human NBPF1 promoter primers, human DTL exon 1 primers, and human ROBO1 promoter primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

KEYWORDS: epigenetic regulation, cancer 

Validated for: WB IP Flow C&R 

Ubiquitin (E6K4Y) XP® Rabbit mAb
#20326

Ubiquitin is a conserved polypeptide unit that plays an important role in 
the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to 
many cellular proteins by the ubiquitination process, which targets 
proteins for degradation by the 26S proteasome. Three components are 
involved in the target protein-ubiquitin conjugation process. 

Keywords: targeted protein degradation, DNA damage and repair, cell 
cycle progression, apoptosis, receptor-mediated endocytosis, and signal 
transduction. 

Validated for: WB IHC