NEB Product update 

RNase R / #M0100 

RNase R (NEB-M0100S) is a highly processive 3′ to 5′ exoribonuclease from E. coli that efficiently digests nearly all linear RNAs with an accessible 3′ end, including rRNA and mRNA. Unlike other exoribonucleases, RNase R is unique in its ability to degrade highly structured RNAs without the need for an additional helicase. RNase R requires a 3′ single-stranded RNA overhang of approximately 10 nucleotides or longer, such as a poly(A) tail, for efficient binding and degradation. 

• Efficient Linear RNA Removal: Rapidly digests linear RNAs, enabling enrichment and validation of circular and lariat RNAs, as these closed RNA structures are resistant to exoribonuclease activity. 
• Flexible Use: Compatible with a wide range of RNA types and workflows, including those for circular RNA therapeutics and diagnostics. 
• Simple Protocol: Incubate at 37°C in 1X RNase R Reaction Buffer; inactivated with EDTA. 

Versatile Applications: Ideal for circular RNA enrichment, validation of circular RNA isolation, and removal of linear RNA in research and manufacturing workflows. 

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Download Product Protocol

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NEB-M0100S 

RNase 4 Digestion and 3′ End Repair Mix / #M1288 

RNase 4 Digestion and 3′ End Repair Mix is a coformulation of RNase 4 and T4 Polynucleotide Kinase, designed to generate a homogeneous pool of RNA oligonucleotides with 3′-hydroxy termini. This combined digestion and 3′ end repair activity simplifies and enhances RNA sequencing coverage analysis and modification mapping by liquid chromatography-mass spectrometry (LC-MS/MS). The mix is available in two sizes: 50 reactions (NEB-M1288S) and 250 reactions (NEB-M1288L). 

• High Specificity and Coverage: Dinucleotide-specific cleavage at U/R (uridine-purine) sites, producing oligonucleotide fragments ideal for LC-MS/MS. 
• Homogeneous 3′ Ends: Coformulation with T4 PNK reduces the 3′ end chemical complexity of digestion products, resulting in a single pool of RNA fragments with 3′-hydroxy ends. 
• Modification Tolerance: Efficiently digests RNAs containing many common uridine modifications, supporting analysis of both natural and synthetic RNAs. 
• Simple Workflow: Convenient, user-friendly protocol—just add 1 μl of mix per reaction, incubate at 37°C, and proceed directly to LC-MS/MS analysis. Reaction can be precisely controlled and stopped with Murine RNase Inhibitor. 
• Versatile Applications: Ideal for nucleotide sequence mapping, detection of RNA modifications, and mRNA cap or poly(A) tail analysis by LC-MS/MS. 

For mRNA 5′ cap analysis use stand-alone RNase 4 (NEB-M1284) with the protocol for DNA Probe-Directed Analysis of mRNA 5´Cap Structures. 

Download Product Brochure 

Download RNase 4 (NEB #M1284) Digestion Protocol 

Download RNase 4 (NEB #M1284) DNA Probe-Directed Analysis of mRNA 5´Cap Structures Protocol 

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NEB-M1288S NEB-M1288L 

NEBNext® Library Quant Kit for Ultima Genomics® / #E3410 

The NEBNext Library Quant Kit for Ultima Genomics is an accurate and streamlined qPCR-based quantitation solution optimized for NGS libraries prepared using the UG 100 Solaris™ Free workflow. The kit is the only qPCR solution specifically designed for the Ultima platform, and it is available in two sizes: 100 reactions NEB-E3410S and 500 reactions (NEB-E3410L). 

• High Performance: Enable quantitation across a broad dynamic range (0.003–30 pM) and compatible with libraries of different sizes (110–700 bp) and GC content. 
• Streamlined Workflow: Single extension time for all libraries irrespective of size, visible tracking dye (no ROX required), and automation-friendly protocol. 
• Flexible and Reliable: Works with PCR-free libraries from different vendors, provided they contain Ultima’s PCR-Free WGS or ppmSeq™ adaptor sequences. Web tool (NEBioCalculator) available for easy calculation and download of quant values. 
• Applications: Accurate qPCR-based quantitation of PCR-free NGS libraries for Ultima Genomics sequencing. Suitable for germline and somatic whole genome sequencing, ppmSeq™ for tumor/normal pairs, liquid biopsy, and MRD applications.  

Download E3410 Product Manual 

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NEB-E3410S NEB-E3410L

NEBExpress® Salt Active Nuclease, GMP Grade / #G8024 

NEBExpress Salt Active Nuclease, GMP Grade is a recombinant, engineered nuclease designed for efficient nucleic acid removal in protein, adeno-associated virus (AAV), and biopharmaceutical manufacturing workflows. The enzyme is available in two sizes: 100,000 units (NEB-G8024S) and 1,000,000 units (NEB-G8024L), with bulk and custom formats also offered. 

• High Performance: Up to 2X more active than other nucleases, NEBExpress Salt Active Nuclease increases productivity and reduces costs in AAV and viral vector bioprocessing.  
• Optimized for High Salt: This enzyme is optimized for high salt conditions, minimizng viral particle aggregation and contamination and enabling superior degradation of all forms of DNA and RNA: double- and single-stranded DNA, RNA, and DNA/RNA hybrids. 
• Fast, Simple Workflow: Formulated at high concentration for direct addition to lysis steps, enabling single-step nucleic acid removal and streamlined purification. 
• Sustainability and Compliance: Recombinant, non-glycosylated, and detergent-free, the enzyme is manufactured in compliance with ISO 9001 and ISO 13485 standards. 
• Versatile Applications: Ideal for AAV and viral vector manufacturing, cell and gene therapy workflows, and any process requiring stringent nucleic acid clearance under GMP-grade conditions. 

Download the G8024 Product Brochure

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NEB-G8024S NEB-G8024L 

Bst-XT WarmStart™ DNA Polymerase / #M9204/M9205L 

Bst-XT WarmStart DNA Polymerase is an engineered variant of Bacillus stearothermophilus DNA Polymerase, Large Fragment, fused to a novel nucleic acid binding domain for enhanced isothermal amplification performance. The polymerase is available in three versions: 1,600 units (NEB-M9204S) and 8,000 units (NEB-M9204L), as well as a glycerol-free version (NEB-M9205L). 

• High Performance: Delivers rapid and robust isothermal amplification for both DNA and RNA targets, matching the speed of Bst 3.0 while maintaining the high specificity of Bst 2.0.  
• Optimized Flexibility: Performs efficiently across a wide temperature range (50–70°C), surpassing the optimal temperature windows of previous Bst polymerases. Suitable for a variety of isothermal amplification applications. 
• Room Temperature Setup: The WarmStart aptamer technology enables room temperature setup by reversibly inhibiting polymerase activity until the reaction is heated. 
• Fast Workflow: Enables detection of 1 ng target DNA within 30 minutes, supporting high-throughput and time-sensitive workflows. 
• Versatile Applications: Ideal for LAMP, RT-LAMP (with companion reverse transcriptase), and other isothermal amplification methods for DNA and RNA detection.  

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NEB-M9204S NEB-M9204L NEB-M9205L

NEBNext® Enzymatic Methyl-seq v2 Kit / #E8015
NEBNext® Enzymatic Methyl-seq v2 Conversion Module / #E8020 

The NEBNext Enzymatic Methyl-seq v2 Kit (EM-seq™ v2) is a high-performance, enzyme-based alternative to bisulfite sequencing for the identification of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in DNA.  The kit includes conversion reagents, library prep reagents, and the EM-seq Adaptor and is available in two versions: 24 rxn (NEB-E8015S) and 96 rxn (NEB-E8015L). Index primers are supplied separately in 96 rxn-format as NEBNext LV Unique Dual Index Primers Set 1, 2, 4, 5 (NEB-E3400S, NEB-E3402S, NEB-E3406S, NEB-E3408S) and 24 rxn-format for Set 2A (NEB-E3390S). 

• High Performance: Delivers superior sensitivity for detecting 5mC and 5hmC, with consistent results across a wide input range (0.1–200 ng). Achieves detection of more CpGs with fewer sequencing reads, even GC coverage, and high-quality library preparation with larger insert sizes. Compatible with established bisulfite data analysis pipelines. 
• Enzyme-Based Conversion: Gentle, two-step enzymatic conversion protects methylated cytosines and selectively deaminates unmethylated cytosines, resulting in minimal DNA damage and improved data quality compared to bisulfite treatment. 
• Fast, Streamlined Workflow: Reduces hands-on time and consumables use, with a total workflow time of approximately 7 hours and multiple safe stopping points. 
• Versatile Applications: Ideal for Illumina sequencing applications, methylome analysis, and any research requiring single-base resolution of DNA methylation.  

The NEBNext Enzymatic Methyl-seq v2 Conversion Module is available for workflows where only the conversion step is required, including non-Illumina sequencing platforms. The module is available in two versions: 24 rxn (NEB-E8020S) and 96 rxn (NEB-E8020L).  

Download Product Manual and Data Supplement for NEBNext® Enzymatic Methyl-seq v2 Kit  
Download Product Manual for NEBNext® Enzymatic Methyl-seq v2 Conversion Module 

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NEB-E8015 NEB-E8020

Monarch® Spin RNA Isolation Kit (Mini) / #T2110 

The Monarch Spin RNA Isolation Kit (Mini) is designed for rapid and reliable isolation, purification, and concentration of high-quality total RNA from a wide variety of biological samples, including cultured cells, mammalian tissues, microbes, plants, insects, and blood. It features upgraded spin columns for low elution volumes and minimal buffer retention, resulting in highly pure RNA suitable for downstream applications. The kit is available in a 50 prep format (NEB-T2110S). 

• High Performance: Achieves high yield, purity, and integrity of total RNA (A260/280 and A260/230 typically >2.0) with minimal residual genomic DNA, capturing RNA from full-length rRNAs down to intact miRNAs. Specialized columns and RNase-free DNase ensure efficient gDNA removal. 
• Low Elution Volume: Elute highly concentrated RNA in as little as 10 μl, supporting applications that require low input or high concentration. 
• Fast Workflow: Comprehensive solution for sample preservation, lysis, gDNA removal, and RNA purification, compatible with a broad range of sample types and storage conditions. 
• Versatile Applications: Purified RNA is ready for downstream molecular applications, such as RT-qPCR, cDNA synthesis, RNA-seq, small RNA library prep, and hybridization-based technologies. 

The T2110 kit retains the user-friendly features of the Monarch product line, such as compatibility with both centrifuges and vacuum manifolds, and introduces significant improvements for superior RNA isolation and purification. 

Download the T2110 Product Manual 

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NEB-T2110 

Monarch® Spin High-Capacity DNA Cleanup Kit / #T1135 

The Monarch Spin High-Capacity DNA Cleanup Kit is designed for rapid and reliable purification and concentration of high-quality DNA from a wide range of sample sources, including genomic DNA, linear DNA, circular DNA, and oligonucleotides. It features a newly engineered spin column for high DNA binding capacity and low elution volumes, resulting in highly pure, concentrated DNA suitable for demanding downstream applications. The kit is available in three versions: 10 preps (NEB-T1135V), 50 preps (NEB-T1135S), and 200 preps (NEB-T1135L). 

• High Performance: Purifies, cleans up, and concentrates up to 100 μg of DNA with high yield and purity, efficiently removing primers, detergents, nucleotides, and other low-molecular-weight contaminants. Suitable for a broad range of DNA fragment sizes, from small oligonucleotides (<40 bp) to large DNA fragments (>25 kb). 
• Low Elution Volume: Elute highly concentrated DNA in as little as 50 μl, supporting workflows that require minimal sample dilution. 
• Fast Workflow: Simple bind/wash/elute protocol compatible with microcentrifuges, centrifuges, and vacuum manifolds for efficient processing. 
• Versatile Applications: Purified DNA is ready for downstream molecular biology applications, such as in vitro transcription (IVT) template preparation, restriction digests, DNA sequencing, ligation, amplification, and other enzymatic reactions. 

The T1135 kit retains the user-friendly features of the Monarch product line, such as compatibility with both centrifuges and vacuum manifolds, and introduces significant improvements in capacity and flexibility for superior DNA cleanup and purification. 

Download #T1135 Product Manual

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NEB-T1135V NEB-T1135S NEB-T1135L 

Monarch® DNase I, Lyophilized / #T2104

Monarch DNase I, Lyophilized (NEB-T2104S) is an RNase-free, lyophilized DNA-specific endonuclease supplied in a dry cake format for maximum stability and convenience. Monarch DNase I efficiently digests single- and double-stranded DNA, chromatin, and RNA:DNA hybrids, making it ideal for removing contaminating genomic DNA from RNA preparations. 

• High Stability: Lyophilized format is stable at room temperature for up to 3 years prior to rehydration, ensuring reliable performance and easy storage. 
• RNase-Free: Formulated to be RNase-free, making it suitable for sensitive RNA workflows. 
• Flexible Use: Can be used on-column or in-solution for optimal DNA removal from RNA samples. 
• Versatile Applications: Ideal for eliminating genomic DNA contamination in RNA samples for downstream applications such as RT-qPCR, RNA-seq, and cDNA synthesis. 

Supplied with DNase I reaction buffer and included as a component in the Monarch Spin RNA Isolation Kit (Mini) (NEB-T2110S), but also available separately for added flexibility. 

Download #T2104 Product Manual

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NEB-T2104S NEB-T2110S

Q5® Quick-Load High-Fidelity 2X Master Mix / #M0578  
Q5® Quick-Load Hot Start High-Fidelity 2X Master Mix / #M0580 

Q5 Quick-Load High-Fidelity 2X Master Mix (NEB-M0578L) is a ready-to-use PCR master mix that combines Q5 High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a robust buffer suitable for a wide range of templates and GC content. The mix includes an inert green tracking dye, enabling direct loading of PCR products onto agarose gels without additional loading buffer—streamlining your workflow and minimizing pipetting steps. 

• High-Fidelity Amplification: Delivers exceptional accuracy and robust amplification, even for templates with high GC content. 
• Convenient Direct Loading: Built-in green tracking dye allows PCR products to be loaded directly onto gels, eliminating the need for a separate loading buffer. 
• Ready-to-Use: All-in-one master mix simplifies reaction setup and reduces the risk of pipetting errors. 
• Versatile Applications: Ideal for routine PCR, cloning, and applications requiring high fidelity and easy gel analysis.  

For increased specificity and prevention of non-specific amplification, Q5 Quick-Load High-Fidelity 2X Master Mix is also available in a Hot Start version (NEB-M0580L), featuring Q5 Hot Start High-Fidelity DNA Polymerase. 

Download #M0578 Product Manual

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NEB-M0578L NEB-M0580L

Cold-Active TEV Protease / #P8118  

Cold-Active TEV Protease (NEB-P8118S) is an engineered version of TEV Protease, optimized for rapid and efficient cleavage of fusion proteins at low temperatures. This highly specific cysteine protease hydrolyzes peptide bonds after the E-N-L-Y-F-Q recognition site and before S/G/A/M/C/H/F, making it ideal for removing affinity tags from recombinant proteins.  

• High Performance at 4 °C: Up to 3× faster than standard TEV protease, with improved stability and minimal autolysis during extended incubations. 
• Broad Compatibility: Active across 4–37 °C, pH 6–9, and up to 2 M NaCl, making it versatile for many protein workflows. 
• Simple Workflow: Features a 7× His-tag for easy removal, can be heat-inactivated after cleavage, and is compatible with common protease inhibitors. 
• Sustainable & Reliable: Recombinant, non-glycosylated, detergent-free, and manufactured under ISO-certified quality standards. 

Versatile Applications: Designed for tag removal from recombinant proteins especially those sensitive to temperature, this enzyme suits workflows in fusion-protein cleavage, protein purification, and structural biology applications under GMP-like conditions where low-temperature processing is critical.  

Download #P8118 Product Manual

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NEB-P8118S