NEB Product update 

NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing /#E7672 

The NEBNext® Companion Module v2 (NEB-E7672) is specifically designed to support the updated Oxford Nanopore Technologies (ONT) SQK-LSK114 ligation sequencing protocols. This module includes essential reagents for DNA repair, end prep, and ligation, tailored to enhance the efficiency and cost-effectiveness of ONT sequencing workflows. It ensures compatibility across all ONT devices, including MinION®, GridION®, PromethION®, and Flongle®.  The kit is available in 2 versions: 24 rxn (NEB-E7672S) and 96 rxn (NEB-E7672L).  

• High Performance: Provides all necessary components for efficient DNA repair and ligation, ensuring high-quality sequencing results with minimal waste. 
• Cost-Effective: Offers a 20% reduction in cost compared to previous versions, with tailored reagent volumes to prevent excess and reduce expenses. 
• Fast Workflow: Streamlined preparation process aligns with ONT protocols, minimizing preparation time and maximizing throughput. 
• Optimized Reagent System: Includes Salt-T4 DNA Ligase and NEBNext® FFPE DNA Repair Buffer v2, ensuring optimal performance in the SQK-LSK114 workflow. 
• Sustainability: Designed to reduce unnecessary buffers and reagents, supporting sustainable laboratory practices. 
• Versatile Applications: Compatible with multiple ONT sequencing devices, providing flexibility for various research and diagnostic applications. 

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 E7672S E7672L

NEBNext® RSV Primer Module / #E9642

The NEBNext® RSV Primer Module (E9642) is designed for the efficient amplification and sequencing of Respiratory Syncytial Virus (RSV) genomes. This module includes two primer mixes optimized for full-length coverage of RSV A and RSV B, making it an essential tool for researchers in virology, public health, and molecular diagnostics. The primers are compatible with both Illumina® and Oxford Nanopore Technologies® sequencing platforms, providing flexibility in downstream applications. The kit is available in 2 versions: 24 rxn (NEB-E9642S) and 96 rxn (NEB-E9642L).  

• High Performance: Achieve comprehensive RSV genome coverage with primers designed to minimize the impact of sequence variants, ensuring reliable and consistent results. 
• Low Elution Volume: Allows for the preparation of highly concentrated DNA libraries, optimizing sequencing efficiency. 
• Fast Workflow: Streamlined process integrates seamlessly with existing workflows, reducing preparation time and increasing throughput. 
• Optimized Buffer System: Includes color indicators for easy monitoring of each step, enhancing accuracy and user confidence. 
• Sustainability: Designed with reduced plastic usage, supporting environmentally conscious laboratory practices. 
• Versatile Applications: Suitable for a wide range of applications, including diagnostic testing, epidemiological studies, and academic research, offering broad utility across various fields. 

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E9642S E9642L

Thermostable Endonuclease Q / #M0701S

Thermostable Endonuclease Q (NEB-M0701S) is an advanced archaeal DNA endonuclease designed to cleave the phosphate backbone of DNA substrates at specific modified nucleobases, including deoxyxanthosine (dX), deoxyinosine (dI), deoxyuracil (dU), and abasic sites. This enzyme is particularly suited for applications requiring site-specific cleavage, offering significant improvements over previous solutions by targeting both single-stranded and double-stranded DNA with a preference for single-stranded substrates. 

• High Performance: Achieve precise cleavage at deaminated nucleobases and abasic sites, leaving a ligatable 3'-OH and a 5'-phosphate, with no other commercially available enzyme offering this specificity. 
• Temperature Versatility: Functions optimally at 65-85°C, with a performance range from 37-90°C, accommodating various experimental conditions. 
• Fast Workflow: Efficiently cleaves DNA substrates, enhancing workflow speed and reliability. 
• Optimized Reaction Conditions: Supplied with 10X NEBuffer r2.1 to ensure optimal enzyme activity and stability during reactions. 
• Sustainability: Packaged in a format designed to minimize waste, supporting environmentally friendly laboratory practices. 
• Versatile Applications: Ideal for enzymatic DNA synthesis, site-specific cleavage in oligonucleotide applications, and more, providing flexibility for diverse research needs. 

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M0701S

phi29-XT WGA Kit #E1604

The phi29-XT WGA Kit (NEB-E1604) is an advanced solution for whole genome amplification (WGA), designed to deliver high yields of DNA from minimal input quantities. Featuring the engineered phi29-XT DNA Polymerase, this kit offers improved thermostability and sensitivity, making it ideal for applications requiring robust amplification from low DNA inputs, such as single-cell and environmental DNA sequencing. The kit is available in 2 versions: 25 rxn (NEB- E1604S) and 100 rxn (NEB-E1604L). 

• High Performance: Efficiently amplifies as little as 10 femtograms of DNA input, providing high yields suitable for various downstream applications. 
• Low Elution Volume: Produces highly concentrated DNA, facilitating further processing and analysis. 
• Fast Workflow: Complete the amplification process in under 2 hours, significantly reducing preparation time. 
• Optimized Buffer System: Includes dNTPs, exonuclease-resistant random primers, and a neutralization buffer, ensuring optimal reaction conditions and high fidelity. 
• Sustainability: Designed to minimize waste, supporting environmentally friendly laboratory practices. 
• Versatile Applications: Suitable for single-cell whole genome sequencing, genomic DNA enrichment, and environmental DNA sequencing, offering flexibility for diverse research needs. 

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E1604S  E1604L

NEBNext Enzymatic 5hmC-seq Kit 

New England Biolabs® EM-seqTM range of products are now expanded by the addition of products for E5hmC-seqTM, which enables specific detection of 5hmC sites. 5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing the determination of the precise location of individual 5mC and 5hmC sites.  

The full workflow requires the NEBNext Enzymatic 5hmC-seq Kit (E3350) plus the index primers included in the NEBNext Primers for Epigenetics products (E3392 and E3404) products. 

The NEBNext Enzymatic 5hmC-seq Conversion Module (E3365) is also available for use in applications beyond Illumina sequencing. 
 

Glycerol-free Alternatives 

WarmStart® RTx Reverse Transcriptase (Glycerol-free) /#M0439

WarmStart RTx Reverse Transcriptase (Glycerol-free) (NEB-M0439) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows. It is the same enzyme as the glycerol-containing WarmStart RTx Reverse Transcriptase (NEB-M0380) product and performs similarly.  

Bst 2.0 WarmStart® DNA Polymerase (Glycerol-free) / #M0402 

Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) (NEB-M0402) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows. It is the same enzyme as the glycerol-containing Bst 2.0 WarmStart DNA Polymerase (NEB-M0538) product and performs similarly.