Bacteria are a diverse group of organisms that come in several different shapes and sizes. In addition, they are highly mobile, are often found in clusters and in different focal planes, which makes quantification a challenge. QUANTOM Tx™ Microbial Cell Counter is an image-based automated cell counter that can identify and count individual bacterial cells in minutes.

Step 1: Staining
QUANTOM™ Total Cell Staining Dye is a green-fluorescent nucleic acid dye that easily penetrates the membranes of both gram positive and gram negative bacteria to stain both live and dead bacterial cells. QUANTOM™ Viable Cell Staining Dye efficiently labels viable bacteria. 

Step 2: Immobilization
Stained bacterial cells are mixed with QUANTOM™ Cell Loading Buffer I, loaded into QUANTOM™ M50 Cell Counting Slides and spun in the QUANTOM™ centrifuge to immobilize and evenly distribute the cells along a single focal plane to ensure accurate cell counting.  

Step 3: Counting
QUANTOM Tx™ takes up to 10 high resolution images and counts cells in each image automatically.