Cell Counting
Cell counting is an important quality control procedure in research. To increase accuracy and convenience, Logos Biosystems has developed broad range of automated cell counters and slides for counting all types of cells and bacteria. In addition we also offer several cell counting reagents.
LUNA-STEM™
Automated Fluorescence Cell Counter
LUNA-II YF™
Automated Yeast Cell Counter
Compare Logos Biosystems cell counters
| LUNA-II™ | LUNA-IIITM | LUNA-FX7™ | LUNA-FL™ | LUNA-STEM™ | LUNA-II YF™ | QUANTOM Tx™ | |
|---|---|---|---|---|---|---|---|
| Main features | Most affordable cell counter | Versatile and affordable cell counter | Versatile cell counter that serves a wide variety of applications. An excellent choice for higher throughput and/or regulatory needs. |
Versatile and affordable fluorescence cell counter with manual focus | Fluorescence cell counter specialized for SVF derived- cells | Affordable yeast cell counter | Image-based bacterial cell counter |
| Cell types | Stable cell lines | Stable cell lines | Cell lines Primary cells Whole blood PBMCs Splenocyte Thymocyte Yeast cells |
Cell lines Primary cells Blood cells PBMCs Splenocyte Thymocyte Yeast cells GFP expressing cells |
Stem cells SVF-derived cells • Nucleated • Non-nucleated |
Yeast cells | Bacterial cells |
| Detection method | Brightfield | Brightfield | Dual fluorescence Brightfield |
Dual fluorescence Brightfield |
Dual fluorescence | Dual fluorescence | Fluorescence |
| Compatible dyes | Trypan blue Erythrosin B |
Trypan blue Erythrosin B |
Trypan blue Erythrosin B Acridine orange Propidium iodide Fluorescein diacetate |
Trypan blue Erythrosin B Acridine orange Propidium iodide Fluorescein diacetate |
Acridine orange Propidium iodide |
Acridine orange Propidium iodide Fluorescein diacetate |
QUANTUM Total Cell Staining Kit QUANTUM Viable Cell Staining Kit |
| Recommended slide options | LUNA™ Cell Counting Slide LUNA™ Reusable Slide |
LUNA™ Cell Counting Slides LUNA™ Reusable Slide |
PhotonSlide LUNA™ 1-Channel Slide LUNA™ 3-Channel Slide LUNA™ 8-Channel Slide |
PhotonSlide™ LUNA™ Reusable Slide |
PhotonSlide™ LUNA™ Reusable Slide |
PhotonSlide™ LUNA™ Reusable Slide |
QUANTOM™ M50 Cell Counting Slide |
| Focusing options | Autofocus Manual focus |
Autofocus Manual focus |
Autofocus Manual focus |
Manual focus | Manual focus | Autofocus Manual focus |
Autofocus Manual focus |
| Counting volume/chamber | 0,5 µl | 0,5 µl | 0,5 - 5,1 µl by slide type | 0,5 µl | 0,5 µl | < 0,1 µl (10 images) | |
| Counting speed | < 10 s (manual focus) < 15 s (autofocus) |
< 10 s (manual focus) < 15 s (autofocus) |
< 10 s (brightfield, without autofocus, 12 images) < 20 s (brightfield, with autofocus, 12 images) < 30 s (fluorescence, without autofocus, 12 images) < 40 s (fluorescence, with autofocus, autofocus, 12 images) |
< 10 s (brightfield) < 30 s (fluorescence) |
< 30 s | < 10 s (manual focus) < 15 s (autofocus) |
< 30 s (autofocus, 10 images) |
| Adjustable protocol parameters | Cell size Declustering Dilution factor Live cell sensitivity Noise reduction Roundness |
Search size / Cell size Cell detection sensitivity Live cell sensitivity Noise reduction Dilution factor |
Search size / Cell size Cell detection sensitivity Live cell sensitivity Noise reduction Fluorescence exposure Fluorescence threshold Dilution factor |
Cell size Dilution factor Fluorescence threshold Live cell sensitivity Noise reduction Roundness |
Cell size Dilution factor Fluorescence threshold Noise reduction Roundness |
Fluorescent object size Dilution factor Fluorescence exposure Light intensity |
Fluorescent object size Dilution factor Roundness Declustering Multi-field imaging |
| Additional features | Cell size gating Cluster map Dilution calculator Review previous count results Auto exposure |
Cell size gating Cluster map Dilution calculator Review previous count results Auto exposure Reanalysis |
Cell size gating Cluster map Dilution calculator Review previous count results Bioprocess Quality control Reanalysis Auto exposure |
GFP expression analysis Cell size gating Cluster map Dilution calculator Review previous count results Auto exposure |
Cell size gating Cluster map Dilution calculator Review previous count results |
Fluorescent object size gating Dilution calculator Review previous count results |
Fluorescent object size gating Dilution calculator Review previous count results Reanalysis |
| IQOQ protocol | Yes | Yes | Yes | Yes | - | Yes | |
| 21 CFR Part 11 ready | - | Yes (With CountWire™ software) | - | - | - | - | |
| Saving options | Cell count and viability data (CSV) Raw image (TIF) Analyzed image (annotated TIF) Full report (PDF) |
Cell count and viability data (CSV) Raw image (TIF) Analyzed image (annotated TIF) Full report (PDF) Bioprocess (Onboard, CSV, PNG graph images) Quality Control (Onboard, CSV, PNG graph images) |
Cell count and viability data (CSV) Raw image (TIF) Analyzed image (annotated TIF) Full report (PDF) |
Cell count and viability data (CSV) Raw image (TIF) Analyzed image (annotated TIF) Full report (PDF) |
Cell count and viability data (CSV) Raw image (TIF) Analyzed image (annotated TIF) Full report (PDF) |
Cell count data (CSV) Raw image (TIF) Analyzed image (annotated TIF) Full report (PDF) |
|
| Data transfer | USB memory stick | USB memory stick, WiFi, Ethernet | USB memory stick | USB memory stick | USB memory stick | USB memory stick, WiFi | |
| Technical specifications | 5” TFT LCD screen 800 X 480 pixels Built-in computer Built-in printer (optional) |
7” TFT LCD screen 1024 X 600 pixels Built-in computer External printer (optional) |
7” TFT LCD screen 800 X 480 pixels Built-in computer External printer (optional) |
7” TFT LCD screen 800 X 480 pixels Built-in computer External printer (optional) |
5” TFT LCD screen 800 X 480 pixels Built-in computer Built-in printer (optional) |
10” TFT LCD screen 1280 x 800 pixels Built-in computer External printer (optional) |
|
| Onboard storage | - | 250GB / 1TB | - | - | - | 128GB | |
| Weight | 1.6 kg | 4.5 kg | 1.8 kg | 1.8 kg | 1.8 kg | 10.8 kg | |
| Dimensions | 16 x 18 x 28 cm | 24.5 x 28 x 24 cm | 22 x 21 x 9 cm | 22 x 21 x 9 cm | 16 x 18 x 28 cm | 43.3 × 31.0 × 22.5 cm |
