Q5 - Fidelity at its Finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (>280 times higher than Taq). Q5 DNA Polymerase results in ultra-low error rates.
Q5 master mixes contain dNTPs, Mg++, and a proprietary broad-use buffer requiring only the addition of primers and DNA templates for robust amplification, regardless of GC content.
The NEBNext master mixes are compatible with and optimized for NGS (Next Generation Sequencing) library preparation, and are included as components in the NEBNext kits for RNA, DNA library, and bisulfite sequencing workflows (NEBNext Q5U Master Mix).
With the new product Q5 Blood Direct, you can run PCR directly on blood samples (up to 30 % whole blood/ EDTA) and even on dried blood spots, without DNA extraction. Well suited for sequences up to 7.5 kb and GC rich sequences with 23-77 % GC.
Comparison of High-Fidelity Polymerases
Order Q5 products from NEB on our webshop
1 NEB continues to investigate improved assays to characterize Q5’s very low error rate to ensure that we present the most accurate fidelity data possible (Potapov, V. and Ong, J.L. (2017) PLoS ONE. 12(1): e0169774).
2 PCR-based mutation screening in lacI (Agilent) or rpsL (Life).
Products included in the offer:
|Product no.||Product name||Size(s)||Order|
|M0491S/L||Q5® High-Fidelity DNA Polymerase||100/ 500 rxns||Order|
|M0492S/L||Q5® High-Fidelity 2X Master Mix||100/ 500 units||Order|
|E0555S/L||Q5® High-Fidelity PCR Kit||50/ 200 rxns||Order|
|M0494S/L/X||Q5® Hot Start High-Fidelity 2X Master Mix||100/ 500/ 500 rxns||Order|
|M0493S/L||Q5® Hot Start High-Fidelity DNA Polymerase||100/ 500 units||Order|
|B9027S||Q5® Reaction Buffer Pack||6 mL||Order|
|M0500S/L||Q5® Blood Direct 2X Master Mix||100/ 500 rxns||Order|
|M0515S/L||Q5U® Hot Start High-Fidelity DNA Polymerase||100/ 500 rxns||Order|
|M0541S/L||NEBNext® High-Fidelity 2X PCR Master Mix||50/ 250 rxns||Order|
|M0543S/L||NEBNext® Q5® Hot Start HiFi PCR Master Mix||50/ 250 rxns||Order|
|M0544S/L/X||NEBNext® Ultra™ II Q5® Master Mix||50/ 250/ 500 rxns||Order|
|M0597S/L||NEBNext® Q5U® Master Mix||50/ 250 rxns||Order|
For more information, please contact:
Marianne Møller Brorson
+45 3140 0171
Terms and conditions: This offer is valid until December 31st, 2022 , and can not be combined with other discounts.