15% off all Biotium products

created on during Q4-22

15% off all Biotium products

Working with Flow Cytometry? check out our supplier Biotium, Glowing products for science  


Biotium is a leading life science reagent manufacturer and supplier devoted to providing high-quality and innovative fluorescent tools. Since its founding in 2001, Biotium has developed over 30 patented technologies, including GelRed® dyes, CF® Dyes, NucView® Caspase-3 Substrates, EvaGreen® Dyes, and TrueBlack® Background Reducers.

Check out Flow Cytometry Reagents from Biotium

Next-Generation Fluorescent Dyes CF® Dyes are a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared (IR) spectrum for labeling biomolecules, especially proteins and nucleic acids. Developed by scientists at Biotium using breakthrough chemistries, CF® Dyes rival or exceed the quality of other commercial dyes, such as Alexa Fluor® dyes, due to several novel features. 


CF® Dye Advantages 

  • Available in over 40 colors, offering the greatest coverage across visible and near-IR wavelengths 

  • Widest selection of spectrally unique dyes to accommodate highly complex panels 

  • CF® Dye antibody conjugates have superior brightness over alternative fluorophores with exceptional signal-to-noise 

Learn more about how CF® Dyes offer advantages for enhanced multiplexing in spectral flow cytometry


Browse products from Biotium here



FAQs about CF Dyes

What does the CF in CF® Dyes stand for?

CF® was initially an abbreviation for “Cyanine-based Fluorescent dyes”. These were the first patented CF® Dyes based on cyanine dye structures. 10 years and more than two dozen dyes later, the CF® Dye portfolio encompasses multiple dye core structures spanning the fluorescence spectrum from UV to near-IR. Today, we believe “CF” more aptly stands for Clear Fluor: dyes that produce superior signal-to-noise

What are pegylated dyes?

Dye pegylation is one of Biotium’s patented dye technologies that improves solubility and brightness of near-infrared dyes without introducing excess negative charge, making our near-IR CF® Dyes industry leaders. See page 3 for more information on pegylated dye technology; see pages 18-19 to learn more about near-IR CF® Dyes.
What are the chemical structures of CF® Dyes? The exact chemical structures of CF® Dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® Dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® Dyes bear the core structures of coumarin, pyrene, rhodamine, or cyanine dyes. Blue fluorescent CF® Dyes are based on a coumarin or pyrene dye core structure, while green to near-IR CF® Dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® Dye invention that makes CF® Dyes superior to other commercial dyes.

















CF dyes










CF® Dyes are available in more than 40 bright fluorescent colors, from blue to near-IR.


Have some extra wall space in your lab? Download & print out the new CF® Dyes for Flow Cytometry Poster from Biotium. This poster is a helpful reference guide for building flow cytometry panels using Biotium’s bright CF® dyes and other commonly used fluorophores. Laser excitations and emission filter sets of typical flow cytometry instrument configurations are included to choose fluorophores for each laser excitation and filter emission range.


Get to know more about Biotium products: 





CF® Dyes 

Next-Generation Fluorescent Dyes  







Cellular Stains 

Selection guide for live and fixed cell staining 







Products for Flow Cytometry






DNA and RNA quantitation



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Our products are for research use only and cannot be delivered to private individuals but only to authorized institutions and businesses.


For further information, please contact:


    Anja FjorbackPlease contact:

    Area Sales Manager
    Anja Fjorback
    +45 2381 1912





Terms and conditions: This offer is valid until March 31st, 2022, and can not be combined with other discounts.