The MycoAlertTM Mycoplasma Detection Kit is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.
Viable mycoplasma in a test sample (cell supernatant) are lysed and the enzymes react with the MycoAlertTM Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferaseenzyme in the MycoAlertTM Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlertTM Substrate (read B), a ratio can be obtained which is indicative of the presence or
absence of mycoplasma.
MycoAlert gives you answers in only 20 minutes!
Lonzas MycoAlert kit detects viable mycoplasma in less than 20 minutes.
A small sample of the cell culture media is used with a two-step protocol (see overview in the figure).
Benefits of the MycoAlert assay include:
- Convenient and fast: Results in <20 min, easy interpretation
- Universal: Confirmed to detect 44 species, predicted to detect most common mollicute contaminators
- Specific: No interference with bacteria, nelible interference with media components
The MycoAlert PLUS Mycoplasma Detection Kit generates a higher light output and has better compatibility with less sensitive plate luminometers compared to the first-generation kit.
MycoAlert™ Assay Control Set. We always recommend using the control set when performing assays. The positive control for the MycoAlert kits is available separately as the MycoAlert™ Assay Control Set. Use 100 μL of MycoAlert™ Buffer or HPLC grade water as negative control.
|Cat. no||Product description||Order|
|LT07-118||MycoAlert Detection Kit 10 tests||Order|
|LT07-218||MycoAlert Detection Kit 25 tests||Order|
|LT07-418||MycoAlert Detection Kit 50 tests||Order|
|LT07-318||MycoAlert Detection Kit 100 tests||Order|
|LT07-701||MycoAlert PLUS detection kit 10 tests||Order|
|LT07-703||MycoAlert PLUS detection kit 30 tests||Order|
|LT07-705||MycoAlert PLUS detection kit 50 tests||Order|
|LT07-710||MycoAlert PLUS detection kit 100 test||Order|
|LT07-518||MycoAlert Assay Control Set (positive control) 10 tests||Order|
For more information, please contact:
Ares Sales Manager
Terms & Conditions:
This offer is valid until June 30th 2023 and can not be combined with other discounts.