10% on new PCR-free NEBNext Kits

created on

10% on new PCR-free NEBNext Kits

 

NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina 

New England Biolabs has developed new kits for PCR-free DNA Library preparation for Next Generation Sequencing on the Illumina platform. The four different newly released NEBNext kits are designed to be applied on either fragmented and non-fragmented DNA and by being provided with or without Sample Purification Beads.

 

The NEBNext Ultra II DNA PCR-free Library Prep Kits to be applied on fragmented DNA contains the enzymes and buffers required to convert a broad range of sheared DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR. Benefit from improved library uniformity and flexible inputs from 250 ng to 1,000 ng of sheared DNA. A version of this kit is available with or without sample purification beads.

 

EBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina® - NEB #E7410 

NEBNext® Ultra™ II DNA PCR-free Library Prep with Sample Purification Beads – NEB #E7415 

 

The NEBNext Ultra II FS DNA PCR-free Library Prep Kit to be applied on non-fragmented DNA contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. In addition, these kits include the FS reagents enabling fragmentation, end repair, and dA-tailing with a single enzyme mix. The fast, user-friendly workflow requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR. Benefit from improved library uniformity and flexible inputs from 50 ng to 500 ng of intact DNA. A version of this kit is available with or without sample purification beads.

 

NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina® - NEB #E7430 

NEBNext® Ultra™ II FS DNA PCR-free Library Prep with Sample Purification Beads – NEB #7435 

 

Use the campaign code NEBPCRFREE10 when ordering

 

Figure 1: NEBNext Ultra II FS DNA PCR-free Library Preparation Kit generates libraries with higher yields
 

Figure 1: NEBNext Ultra II FS DNA PCR-free Library Preparation Kit generates libraries with higher yields

 


A. PCR-free libraries were prepared with human cell line NA19240 genomic DNA (Corielle Institute) using NEBNext Ultra II FS DNA PCR-free and Illumina PCR-free library prep kits and size selected for 350 bp long inserts.

B. Libraries of 150-200 bp inserts were prepared using the enzyme-based fragmentation kits NEBNext Ultra II FS DNA PCR-free and Roche Sequencing Kapa HyperPlus PCR-free library prep kits without size selection. NEBNext Unique Dual Index UMI Adaptors DNA Set 1 and Kapa Dual-Indexed Adaptors were used for the NEBNext and Kapa kits, respectively, following manufacturers' recommendations.

Figure 2: NEBNext Ultra II FS DNA PCR-free Library Prep provides uniform genome coverage with human DNA
 

NEBNext Ultra II FS DNA PCR-free libraries have more even genome coverage than Illumina DNA PCR-free. Sequencing libraries were prepared according to the manufacturer's recommendations from 250 ng of NA19240 cell line DNA by 2 different users and sequenced on a single NovaSeq® 6000 S4 v1.5 flow cell (PE 2x150 bp). Reads were adaptor trimmed (fastp 0.20.0), aligned to the Telomere-to-Telomere chm13 references (draft 1, bwa-mem 0.7.17), and duplicate marked (samblaster 0.1.24). 2.8B reads were randomly sampled from each bam (sambamba view -s 0.6.8). Coverage of primary, pass-filter assignments was assigned using mosdepth (v0.3.1, -F 772) and plotted for autosomes or the mitochondrial genome (inset). Expected coverage (num_reads*read length/genome size) is indicated. There is significant variation between users for Illumina PCR-free libraries; however, the FS DNA PCR-free libraries are very consistent between users.

For more information, inquiries or prices:
 

   

 

   Scientific Application Specialist
   Marianne Møller Brorson
   +45 3140 0171
   mmb@bionordika.dk

 

 

   E7410/E7415 Protocol

 

 

 

 

     

   E7430/7435 Protocol


 


TERMS AND CONDITIONS: This offer is valid until March 31st 2022 and can not be combined with other discounts.
Use the campaign code NEBPCRFREE10 when ordering. BioNordika accepts no liability for any typing errors.