CUT Your Chromatin Profiling Time with CUT&Tag from CST®
17. July 2024
Want to understand DNA-protein interactions but don't have the time to perform ChIP-seq? Meet CUT&Tag (Cleavage Under Targets & Tagmentation), the newest member of our chromatin profiling portfolio that delivers:
- Time-savings: Profile chromatin 25% faster than CUT&RUN.
- Sample-savings: Use ~40x less sample compared to ChIP-seq.
- Cost-savings: Run more samples per sequencing lane because CUT&Tag is a low background assay and requires lower sequencing depths.
CUT&Tag Reagents Just the Way You Need Them
Order the CST® CUT&Tag Assay Kit #77552 to get all the reagents you need with a few clicks. Or purchase items separately from our CUT&Tag product portfolio, such as the CST CUT&Tag pAG-Tn5 (loaded) #79561.
Want to know more, download the flyer! Chromatin Profiling Just Got Faster with CUT&Tag
CUT&Tag: An Introduction
Cleavage Under Targets & Tagmentation (CUT&Tag), like CUT&RUN, is a new chromatin profiling method that requires less of your sample and time. It uses a primary antibody, a secondary antibody, and pAG-Tn5 to identify protein-DNA interactions across the genome for analysis with next-generation sequencing (NGS). A major advantage of CUT&Tag is that the Tn5 also completes the adaptor ligation as part of the assay, which streamlines the DNA library prep.
We have compiled a collection of videos, application notes, eBooks, blogs, and more to help you start using CUT&Tag to profile histones, transcription factors, and cofactors. Go from cells to DNA library with as little as 100,000 cells in just 1-2 days.
Check out the CST CUT&Tag Resource Center for FAQs, product information, protocols and more!
Webinar: Chromatin Profiling Just Got Faster with CUT&Tag
Watch the On-demand webinar and Q&A with Fang Chen, PhD, Associate Director of Epigenetics Applications at Cell Signaling Technology. Dr Chen will delve into CUT&Tag, a novel chromatin profiling technique, its unique ability to unravel protein-DNA interactions across the genome, and how it can help fuel your research.
In this session, you'll learn:
- How CUT&Tag works and its time-and cost-saving benefits
- What to consider when deciding between CUT&Tag and CUT&RUN for your specific experimental needs
- How CST ensures the performance of all CUT&Tag products
Let's talk Science! Reach out for more information!
